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All subjects (759 acute ischemic stroke patients and 786 controls) were genotyped using ligation detection reaction (LDR). To investigate the relationship between single nucleotide polymorphism ( SNP) rs6007897 of CELSR 1 and acute ischemic stroke in Western China Han population. Qin, Feng-qin Yu, Li-hua Hu, Wen-ting Guo, Jian Chen, Ning Guo, Jiang Fang, Jing-huan He, Li We were unable to confirm the suggested favorable outcome of SNP rs16754 in de novo AML. Both techniques are equally reliable and reproducible as screening methods for the detection of the SNP rs16754, allowing for the selection of those samples that will need to be sequenced. No other difference was observed for baseline characteristic or outcome between patients with or without the SNP. Patients with the wild-type genotype (WT 1(AA)) for the SNP rs16754 were significantly younger than those with the heterozygous WT 1(AG) genotype. Direct sequencing confirmed that every positive sample in the screening methods had a variation in the DNA sequence. The SNP was detected in 52 out of 175 patients (30 %), both by HRM and hybridization probes. Direct sequencing was used to validate both techniques.

One hundred and seventy-five adult de novo AML patients were screened with two different methods for the detection of SNP rs16754: high-resolution melting (HRM) and FRET hybridization probes. Nevertheless, the findings in this field are not always reproducible in different series. The single nucleotide polymorphism ( SNP) rs16754 of the WT 1 gene has been previously described as a possible prognostic marker in normal karyotype acute myeloid leukemia (AML) patients. Luna, Irene Such, Esperanza Cervera, Jose BarragÃ¡n, Eva JimÃ©nez-Velasco, Antonio Dolz, Sandra IbÃ¡Ã±ez, Mariam GÃ³mez-SeguÃ, InÃ©s LÃ³pez-PavÃa, MarÃa Llop, Marta Fuster, Ã“scar Oltra, Silvestre MoscardÃ³, Federico MartÃnez-CuadrÃ³n, David Senent, M Leonor GascÃ³n, Adriana Montesinos, Pau MartÃn, Guillermo Bolufer, Pascual Sanz, Miguel A

Analysis of SNP rs16754 of WT 1 gene in a series of de novo acute myeloid leukemia patients.

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